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从成年小鼠肾脏中分离细胞核以进行单核RNA测序

JoVE Journal
Biologie

This content is Free Access.

JoVE Journal Biologie

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

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从成年小鼠肾脏中分离细胞核以进行单核RNA测序

3,779 Views

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06:00 min

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September 20, 2021

DOI:

10.3791/62901-v

DOI:

10.3791/62901-v

•

06:00 min

September 20, 2021

•

13 Views

Janna Leiz*^1,2, Christian Hinze*^1,2, Anastasiya Boltengagen³, Caroline Braeuning⁴, Christine Kocks³, Nikolaus Rajewsky³, Kai M. Schmidt-Ott^1,2

¹Department of Nephrology and Medical Intensive Care Medicine,Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, ²Molecular and Translational Kidney Research,Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), ³Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements,Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), ⁴Berlin Institute of Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)

Summary

在这里，我们提出了一种从冷冻小鼠肾脏中分离高质量细胞核的方案，该方案改善了髓质肾细胞类型的表征，并避免了酶组织解离引起的基因表达伪像。

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