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Biotechnik
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基于莱萨特的无细胞系统中的代谢物分析液色谱耦合折射指数或质谱检测
JoVE Journal
Biotechnik
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JoVE Journal
Biotechnik
Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Please note that all translations are automatically generated.
Click here for the English version.
基于莱萨特的无细胞系统中的代谢物分析液色谱耦合折射指数或质谱检测
DOI:
10.3791/62852-v
•
14:42 min
•
September 23, 2021
•
Jaime Lorenzo N. Dinglasan
3
,
David T. Reeves
3
,
Robert L. Hettich
,
Mitchel J. Doktycz
1
Graduate School of Genome Science & Technology
,
University of Tennessee Knoxville
,
2
Bredesen Center for Interdisciplinary Research
,
University of Tennessee Knoxville
,
3
Biosciences Division
,
Oak Ridge National Laboratory
Kapitel
00:04
Introduction
01:08
Starting, Stopping, and Processing Time Course CFME Reactions for HPLC-RID Quantification
03:18
Creating a Sequence Table for Autosampling and Start the HPLC-RID System for Data Acquisition
04:54
Extracting and Analyzing Data Post-Run
07:20
Starting, Stopping, and Processing Time Course Isotope Tracing CFME Reactions for LC-MS/MS Quantification
09:20
Setting Up a Run Sequence and Starting the LC-MS/MS Run
10:16
Calculating Negative Mode Masses of 13C-Labeled Glucose-Derived Metabolites and Searching for the m/z Features of These Analytes in Filtered Data
11:45
Results: Metabolite Profiling in Lysate-based Cell-free Systems
13:54
Conclusion
Summary
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Automatische Übersetzung
协议描述了高性能液相色谱方法,以及用于研究复杂赖酸基无细胞系统代谢反应的折射指数或质谱检测。
Tags
Liquid Chromatography
Refractive Index Detection
Mass Spectrometry
Metabolite Profiling
Cell-free Systems
Carbon Metabolism
Central Metabolites
Lysate-based
HPLC
Sample Preparation
Acidification
Filtration
Article
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