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全血とバフィーコートからのヒト好中球の分離
JoVE Journal
Biologie
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JoVE Journal Biologie
Isolation of Human Neutrophils from Whole Blood and Buffy Coats
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全血とバフィーコートからのヒト好中球の分離

15,649 Views

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08:12 min

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September 17, 2021

DOI:

10.3791/62837-v

DOI:
10.3791/62837-v

•

08:12 min
September 17, 2021

•

68 Views
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1Department of Pathology,Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, 2Department of Microbiology, Faculty of Science,Mahidol University

Summary

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全血、バフィーコート、または白血病膜からの好中球の分離方法を詳述し、良好な収率、高純度、および最小限の細胞活性化を達成する。我々は、精製の勾配、赤血球(RBC)沈殿、RBC溶菌を利用して、高品質/純度好中球製剤を得る。

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Cite this Article

Hsu, A. Y., Peng, Z., Luo, H., Loison, F. Isolation of Human Neutrophils from Whole Blood and Buffy Coats. J. Vis. Exp. (175), e62837, doi:10.3791/62837 (2021).

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