Journal
/
Immunologie und Infektion
/
用户友好型、高通量和全自动数据采集软件，用于单颗粒冷冻电子显微镜
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
Please note that all translations are automatically generated. Click here for the English version.

用户友好型、高通量和全自动数据采集软件，用于单颗粒冷冻电子显微镜

3,161 Views

•

07:56 min

•

July 29, 2021

DOI:

10.3791/62832-v

DOI:
10.3791/62832-v

•

07:56 min
July 29, 2021

•

16 Views
, , ,
1Molecular Biophysics Unit,Indian Institute of Science, 2Gatan Inc.

Summary

Automatically generated

单颗粒冷冻电子显微镜需要合适的软件包和用户友好的管道来实现高通量自动数据采集。在这里，我们介绍了全自动图像采集软件包Latitude-S的应用，以及用于在低剂量条件下收集玻化生物分子数据的实用管道。

Explore More Videos

1. Automated Data Acquisition Software2. Single-particle Cryo-electron Microscopy3. Structural Characterization4. Biological Macromolecules5. Pathogen Biology6. Latitude-S7. Technique Manager8. Single-Particle Automated Data Collection9. TEM Condition10. Camera Condition11. Atlas State12. Grid State13. Hole State14. Focus State15. Data State16. Defocus Values17. Microscope Settings18. Imaging Optics19. Illumination Conditions20. Camera Exposure Time

Read Article

Cite this Article

Kumar, A., P., S., Gulati, S., Dutta, S. User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy. J. Vis. Exp. (173), e62832, doi:10.3791/62832 (2021).

Download .ris file

Copy

Share Video

.

Copy