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体外荧光显微镜基于动感检测的肌素特异性适应
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Biochemie
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JoVE Journal Biochemie
Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays
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体外荧光显微镜基于动感检测的肌素特异性适应

5,410 Views

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08:57 min

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February 04, 2021

DOI:

10.3791/62180-v

DOI:
10.3791/62180-v

•

08:57 min
February 04, 2021

•

9 Views
, , , ,
1Laboratory of Molecular Physiology, Cell and Developmental Biology Center, National Heart, Lung and Blood Institute,National Institutes of Health, 2Department of Physiology, Perelman School of Medicine,University of Pennsylvania

Summary

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这里介绍的是一个过程来表达和净化肌素5a，然后讨论其特征，使用合奏和单分子体外荧光显微镜为基础的测定，以及如何修改这些方法来描述非肌肉肌素2b。

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Cite this Article

Tripathi, A., Bond, C., Sellers, J. R., Billington, N., Takagi, Y. Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays. J. Vis. Exp. (168), e62180, doi:10.3791/62180 (2021).

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