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开源微型氟度计,以监测资源有限设置中的实时异热核酸放大反应
JoVE Journal
Biotechnik
This content is Free Access.
JoVE Journal
Biotechnik
Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
Please note that all translations are automatically generated.
Click here for the English version.
开源微型氟度计,以监测资源有限设置中的实时异热核酸放大反应
DOI:
10.3791/62148-v
•
09:36 min
•
February 03, 2021
•
Jackson Coole*
1
,
Alex Kortum*
1
,
Yubo Tang
,
Imran Vohra
,
Yajur Maker
,
Kathryn Kundrod
,
Mary Natoli
,
Rebecca Richards-Kortum
1
Department of Bioengineering
,
Rice University
Kapitel
00:04
Introduction
00:44
Optical Housing Assembly
03:39
Electronics and Touchscreen Assembly
06:46
Real-Time Fluorescence Data Recording
08:08
Results: Representative Miniature Fluorimeter Measurements
09:07
Conclusion
Summary
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Automatische Übersetzung
提供详细的说明,以建立一个开源的模块化氟度计,与许多低成本加热器兼容,以执行实时,定量等热核酸放大。
Tags
Keyword Extraction:Open-source
Miniature
Fluorimeter
Isothermal
Nucleic Acid Amplification
Resource-limited Settings
Molecular Diagnostics
Isothermal Amplification
Optics Enclosure
Test Board
Focal Length Lens
Long Pass Filter
Emission Filter
Dichroic Mirror
Beam Splitter
Excitation Filter
LED
Article
Embed
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