/

/

大规模培育三维重建人类表皮

JoVE Journal
Biotechnik

This content is Free Access.

JoVE Journal Biotechnik

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Please note that all translations are automatically generated. Click here for the English version.

大规模培育三维重建人类表皮

9,516 Views

•

08:49 min

•

May 28, 2021

DOI:

10.3791/61802-v

DOI:

10.3791/61802-v

•

08:49 min

May 28, 2021

•

37 Views

Irini M. Dijkhoff¹, Benedetta Petracca^1,2, Roxane Prieux³, Giuseppe Valacchi^3,4, Barbara Rothen-Rutishauser¹, Marc Eeman²

¹Adolphe Merkle Institute,University of Fribourg, ²Dow Silicones Belgium SRL, ³Department of Biomedical and Specialist Surgical Sciences,University of Ferrara, ⁴Department of Animal Sciences, Plants for Human Health Institute,North Carolina State University

Summary

该协议描述了一种简单明了的方法，以可复制和稳健的方式培养三维重组的人类表皮。此外，它还特征了表皮屏障模型的结构-功能关系。还介绍了重组后的人类表皮对前燃刺激的生物学反应。

Explore More Videos

3D Epidermal Model Reconstructed Human Epidermis (RHE)Normal Human Epidermal Keratinocytes (NHEKs)Cell Culture In Vitro Large-scale Cultivation Cell Seeding Cell Passaging Trypsinization Cell Suspension Submerged Culture Air-liquid Interface Culture

Read Article