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Journal
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Immunologie und Infektion
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使用全身吸入系统在小鼠中暴露香烟烟雾
JoVE Journal
Immunologie und Infektion
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JoVE Journal
Immunologie und Infektion
Cigarette Smoke Exposure in Mice using a Whole-Body Inhalation System
Please note that all translations are automatically generated.
Click here for the English version.
使用全身吸入系统在小鼠中暴露香烟烟雾
DOI:
10.3791/61793-v
•
06:07 min
•
October 22, 2020
•
Daniel E. Morales-Mantilla
2
,
Xinyan Huang
7
,
Philip Erice
3
,
Paul Porter
,
Yun Zhang
5
,
Mary Figueroa
,
Joya Chandra
,
Katherine Y. King
,
Farrah Kheradmand
8
,
Antony Rodríguez
8
1
Program in Immunology
,
Baylor College of Medicine
,
2
Department of Pediatrics, Section of Infectious Diseases
,
Baylor College of Medicine
,
3
Department of Medicine – Immunology Allergy and Rheumatology
,
Baylor College of Medicine
,
4
Department of Medicine, Pulmonary, Critical Care, Sleep Medicine
,
Baylor College of Medicine
,
5
Department of Pathology and Immunology
,
Baylor College of Medicine
,
6
Department of Pediatrics, Research and Department of Epigenetics and Molecular Carcinogenesis
,
The University of Texas MD Anderson Cancer Center
,
7
Division of Pulmonary and Critical Care Medicine
,
The First Affiliated Hospital of Sun Yat-sen University
,
8
Center for Translational Research on Inflammatory Diseases (CTRID)
,
Michael E. DeBakey VA Medical Center
Kapitel
00:04
Introduction
00:34
Setting Pressure and Airflow
01:46
Cigarette Smoke Treatment
03:37
Results: CS-mediated lnduction of Airway lnflammation and Lung Emphysematous Changes in Mice
05:14
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
该协议展示了对香烟烟雾(CS)的病理生理影响的研究,内部内建立了全身吸入(WBI)暴露系统(WBIS)。该系统可以在受控的可重复条件下使动物接触CS,用于研究CS介于肺气肿和血细胞瘤的影响。
Tags
Cigarette Smoke Exposure
Whole-body Inhalation System
Chronic Airway Inflammation
Emphysema
Bone Marrow Immune Cell Development
Air Compressor
Flow Meter
Valve Controller
Exposure Chambers
Biological Safety Cabinet
Cigarette Chamber
Timer Settings
Smoke Exposure
Article
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