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Immunology and Infection

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JoVE Journal Immunology and Infection
Cigarette Smoke Exposure in Mice using a Whole-Body Inhalation System
 

Cigarette Smoke Exposure in Mice using a Whole-Body Inhalation System

Article DOI: 10.3791/61793-v • 06:07 min • October 22nd, 2020
October 22nd, 2020 •
1,2, 3,7, 1,3, 4, 1,5, 6, 6, 2, 4,8, 3,8
1Program in Immunology, Baylor College of Medicine, 2Department of Pediatrics, Section of Infectious Diseases, Baylor College of Medicine, 3Department of Medicine - Immunology Allergy and Rheumatology, Baylor College of Medicine, 4Department of Medicine, Pulmonary, Critical Care, Sleep Medicine, Baylor College of Medicine, 5Department of Pathology and Immunology, Baylor College of Medicine, 6Department of Pediatrics, Research and Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, 7Division of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Sun Yat-sen University, 8Center for Translational Research on Inflammatory Diseases (CTRID), Michael E. DeBakey VA Medical Center

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This protocol demonstrates the study of the pathophysiologic effects of cigarette smoke (CS) with a whole-body inhalation (WBI) exposure system (WBIS) built in-house. This system can expose animals to CS under controlled repeatable conditions for research of CS-mediated effects on lung emphysema and hematopoiesis.

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Cigarette Smoke Exposure Mice Whole-body Inhalation System Chronic Airway Inflammation Emphysema Bone Marrow Immune Cell Development Low Cost Building Procedure Labs Air Compressor Pressure Regulator Flow Meter Valve Controller Digital Timer Pulse C Operating Mode Set Lock Key Up Key Reset Key Working Mode T1 Timer T2 Timer Exposure Chambers
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