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Uso de neurônios hipocampais primários para estudar a montagem de segmentos iniciais de Axon
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JoVE Journal Neurowissenschaften
Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments

Uso de neurônios hipocampais primários para estudar a montagem de segmentos iniciais de Axon

4,827 Views

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06:53 min

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February 12, 2021

DOI:

10.3791/61411-v

DOI:
10.3791/61411-v

•

06:53 min
February 12, 2021

•

12 Views
,
1School of Basic Medical Sciences,Xi’an Jiaotong University, 2Department of Cell Biology,Duke University, 3Department of Biochemistry,Duke University

Summary

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Aqui, descrevemos um protocolo para estudar quantitativamente a montagem e estrutura dos segmentos iniciais de axônio (AIS) de neurônios hipocampais que carecem de AIS pré-montada devido à ausência de um anquilírin-G gigante.

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Cite this Article

Yang, R., Bennett, V. Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments. J. Vis. Exp. (168), e61411, doi:10.3791/61411 (2021).

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