Journal
/
Entwicklungsbiologie
/
In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells
JoVE Journal
Entwicklungsbiologie
This content is Free Access.
JoVE Journal Entwicklungsbiologie
In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells

In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells

DOI:
10.3791/61396-v

•

06:01 min

•

December 03, 2020

•

, , ,
1Department of Clinical Application, Center for iPS Cell Research and Application (CiRA),Kyoto University, 2Department of Pediatrics,Kyoto Prefectural University of Medicine, 3Graduate Institute of Clinical Medicine,Taipei Medical University

Kapitel

  • 00:04Introduction
  • 00:59Induced Pluripotent Stem Cell (iPSC) Culture
  • 02:00iPSC Differentiation
  • 03:04Neuromuscular Junction (NMJ) Muscle Contraction and Curare Treatment
  • 03:47Results: Representative NMJ Phenotypic, Morphological, and Functional Characterization
  • 05:20Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here we provide a protocol to generate in vitro NMJs from human induced pluripotent stem cells (iPSCs). This method can induce NMJs with mature morphology and function in 1 month in a single well. The resulting NMJs could potentially be used to model related diseases, to study pathological mechanisms or to screen drug compounds for therapy.

Tags

Human Induced Pluripotent Stem CellsNeuromuscular JunctionMotor NeuronsSkeletal MuscleSchwann CellsSpinal Muscular AtrophyCell DifferentiationCell CultureCell DetachmentExtracellular MatrixDoxycyclineDifferentiation MediumMyogenic Differentiation MediumNeuromuscular Junction Medium

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Read Article