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Isolamento dei nuclei di tessuti adiposi per applicazioni genomiche a singola cellula
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JoVE Journal Genetik
Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications
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Isolamento dei nuclei di tessuti adiposi per applicazioni genomiche a singola cellula

9,589 Views

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07:03 min

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June 12, 2020

DOI:

10.3791/61230-v

DOI:
10.3791/61230-v

•

07:03 min
June 12, 2020

•

11 Views
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1Departments of Medicine,Albert Einstein College of Medicine, 2Departments of Molecular Pharmacology,Albert Einstein College of Medicine, 3Fleischer Institute of Diabetes and Metabolism

Summary

Automatically generated

Questa pubblicazione descrive un protocollo per l'isolamento dei nuclei dagli adipociti maturi, la purificazione mediante lo smistamento attivato dalla fluorescenza e la trascrittomica a livello di cellula.

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Cite this Article

Benitez, G. J., Shinoda, K. Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications. J. Vis. Exp. (160), e61230, doi:10.3791/61230 (2020).

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