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Indução de lesão cerebral axonal difusa em ratos com base na aceleração rotacional
JoVE Journal
Neurowissenschaften
Author Produced
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JoVE Journal Neurowissenschaften
Induction of Diffuse Axonal Brain Injury in Rats Based on Rotational Acceleration
DOI:

06:14 min

May 09, 2020

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Kapitel

  • 00:04Introduction
  • 00:45Preparing Rats for the Experimental Procedure
  • 01:11Procedure of Induction Diffuse Axonal Brain Injury
  • 01:54Measurement of Rotational Kinematics, Biomechanical Parameters
  • 02:32Evaluation of Neurological Severity Score after 48 hours
  • 03:08Brain Collection for Histological Examination after 48 hours
  • 04:02Immunohistochemical Staining
  • 04:16Ergebnisse
  • 05:22Conclusion

Summary

Automatische Übersetzung

Este protocolo valida um modelo de roedor confiável, fácil de executar e reprodutível da lesão axonal difusa cerebral (DAI) que induz danos generalizados de matéria branca sem fraturas no crânio ou contusões.

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