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A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases
1Barbara Davis Center for Diabetes,University of Colorado School of Medicine, 2Department of Endocrinology, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine,Chinese Academy of Medical Sciences, 3Department of endocrinology, Guangzhou First People’s Hospital, School of Medicine,South China University of Technology
Kapitel
- 00:04Introduction
- 00:50Mixed Linker-Coupled Antigen Solution
- 02:26Serum Sample Incubation with Labeled Antigen
- 03:30Multiplex Plate Preparation
- 03:57Serum/Antigen Incubate Transfer
- 04:58Results: Representative Autoantibody Level Evaluation
- 06:03Conclusion
We model a simple multiplexed ECL assay that combines 7 autoantibody assays together. The assay is capable of screening for T1D and multiple other autoimmune diseases, simultaneously, including celiac disease, autoimmune thyroid disease, and autoimmune polyglandular syndrome 1.
