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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
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JoVE Journal Chemie
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

22,070 Views

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11:00 min

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May 20, 2013

DOI:

10.3791/50433-v

DOI:
10.3791/50433-v

•

11:00 min
May 20, 2013

•

18 Views
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1Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, Department of Pharmacology,University of Pennsylvania

Summary

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Untargeted metabolomics provides a hypothesis generating snapshot of a metabolic profile. This protocol will demonstrate the extraction and analysis of metabolites from cells, serum, or tissue. A range of metabolites are surveyed using liquid-liquid phase extraction, microflow ultraperformance liquid chromatography/high-resolution mass spectrometry (UPLC-HRMS) coupled to differential analysis software.

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Untargeted MetabolomicsUPLC-HRMSLiquid-liquid ExtractionPolar And Non-polar MetabolitesMicroflow UPLCPositive And Negative IonizationLabel-free Differential AnalysisMetabolic Pathway ScreeningBiomarker DiscoveryDrug Development

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Snyder, N. W., Khezam, M., Mesaros, C. A., Worth, A., Blair, I. A. Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS). J. Vis. Exp. (75), e50433, doi:10.3791/50433 (2013).

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