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In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time
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1Department of Pulmonary Diseases, Amsterdam UMC,VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS), 2BIOS Lab-on-a-Chip group,University of Twente, 3Applied Stem Cell Technologies Group,University of Twente, 4Department of Cardio-thoracic Surgery, Amsterdam UMC,VU University Medical Center, 5Institute of Physiology,Charité-Universitätsmedizin, 6German Heart Center
Kapitel
- 00:04Introduction
- 00:59Primary Human Pulmonary Arterial Endothelial Cell (PAEC) Isolation and Culture
- 02:43Flow Chambers and PAEC Monolayer Preparation
- 04:00Washed Human Whole Blood Preparation
- 04:50Flow System Assembly
- 05:41Washed Whole Blood Perfusion
- 06:44Results: Representative Thrombus Formation Characterization
- 08:29Conclusion
Presentamos un modelo de enfermedad vascular in vitro para investigar interacciones sanguíneas enteras con endotelio derivado del paciente. Este sistema permite el estudio de las propiedades trombogénicas de las células endoteliales primarias en diversas circunstancias. El método es especialmente adecuado para evaluar la trombogenicidad in situ y la terapia de anticoagulación durante diferentes fases de la coagulación.
