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使用基因组-规模CRISPR/Cas9基因屏幕进行细胞表面受体识别
JoVE Journal
Genetik
This content is Free Access.
JoVE Journal
Genetik
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
Please note that all translations are automatically generated.
Click here for the English version.
使用基因组-规模CRISPR/Cas9基因屏幕进行细胞表面受体识别
DOI:
10.3791/60803-v
•
08:49 min
•
June 06, 2020
•
Sumana Sharma
2
,
Gavin J. Wright
1
Cell Surface Signalling Laboratory
,
Wellcome Trust Sanger Institute
,
2
EMBL-EBI
,
Wellcome Genome Campus
Kapitel
00:04
Introduction
01:07
Quantification and Oligomerization of Monomeric Biotinylated Protein
03:26
Genetic Screening for Cell Surface Binding
05:13
Bioinformatics Analysis to Identify the Receptor and Related Pathways
06:05
Results: Genetic Screens for the Identification of Cell Surface Binding Partners
07:57
Conclusion
Summary
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Automatische Übersetzung
本手稿描述了一种基于基因组规模的细胞筛选方法,用于识别细胞外受体-配体相互作用。
Tags
Cell Surface Receptor
CRISPR/Cas9
Genetic Screens
Cell Signaling
Cell Recognition
Receptor-ligand Interactions
Cell Surface Molecules
Therapeutic Implications
High-throughput Cell Sorting
Next-generation Sequencing
Biotinylated Proteins
Streptavidin-PE
Dilution Buffer
Wash Buffer
Alkaline Phosphatase Conjugate
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