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Quantitative Measurement of Intrathecally Synthesized Proteins in Mice
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JoVE Journal Neurowissenschaften
Quantitative Measurement of Intrathecally Synthesized Proteins in Mice

Quantitative Measurement of Intrathecally Synthesized Proteins in Mice

DOI:
10.3791/60495-v

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08:23 min

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November 29, 2019

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, , , , ,
1Department of Neurology,Geisel School of Medicine & Dartmouth-Hitchcock Medical Center, 2Program in Experimental and Molecular Medicine,Dartmouth College

Kapitel

  • 00:04Titel
  • 01:18Serum Collection: Survival Procedure
  • 02:44CerebroSpinal Fluid (CSF) Collection: Survival Procedure
  • 05:05Serum and CSF Collection: Non-Survival Procedure
  • 06:07Protein Analysis
  • 07:02Results: Representative Blood-Brain Barrier Function and Intrathecal IgG Synthesis in Relapsing Experimental Autoimmune Encephalomyelitis (R-EAE) and Theiler’s Murine Encephalomyelitis Virus-Induced Demyelinating Disease (TMEV-IDD)
  • 07:53Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Elevated spinal fluid protein levels can either be the result of diffusion of plasma protein across an altered blood-brain barrier or intrathecal synthesis. An optimized testing protocol is presented in this article that helps to discriminate both cases and provides quantitative measurements of intrathecally synthesized proteins.

Tags

Keywords: Quantitative MeasurementIntrathecally Synthesized ProteinsMouse ModelsNeurological DisordersCSF Protein LevelsBlood-CSF Barrier IntegrityCentral Nervous System DiseasesRetro-orbital BleedingSerum CollectionCerebral Spinal Fluid Collection

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