Journal
/
Umwelt
/
Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds
JoVE Journal
Umwelt
Author Produced
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Umwelt
Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds

Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds

6,096 Views

•

12:02 min

•

August 08, 2020

DOI:

10.3791/60462-v

DOI:
10.3791/60462-v

•

12:02 min
August 08, 2020

•

6 Views
, , , , , , , , ,
1Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences,Szent István University, 2Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences,University of Birmingham

Summary

Automatically generated

Present here is a detailed protocol for the use of zebrafish embryos Tg(vtg1: mCherry) for the detection of estrogenic effects. The protocol covers the propagation of the fish and treatment of embryos, and emphasizes the detection, documentation, and the evaluation of fluorescent signals induced by endocrine disrupting compounds (EDC).

Explore More Videos

Tg(Vtg1:mcherry) ZebrafishEndocrine Disrupting CompoundsEstrogenic EffectsBiomarkerVitellogeninIn Vivo TestingEnvironmental SamplesAnimal Welfare

Read Article

Cite this Article

Csenki, Z., Horváth, Á., Bock, I., Garai, E., Kerekes, F., Vásárhelyi, E., Kovács, B., Urbányi, B., Mueller, F., Bakos, K. Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds. J. Vis. Exp. (162), e60462, doi:10.3791/60462 (2020).

Download .ris file

Copy

Share Video

.

Copy