Journal
/
环境科学
/
Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds
JoVE 杂志
环境科学
Author Produced
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 环境科学
Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds

Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds

6,096 Views

•

12:02 min

•

August 08, 2020

DOI:

10.3791/60462-v

DOI:
10.3791/60462-v

•

12:02 min
August 08, 2020

•

6 Views
, , , , , , , , ,
1Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences,Szent István University, 2Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences,University of Birmingham

Summary

Automatically generated

Present here is a detailed protocol for the use of zebrafish embryos Tg(vtg1: mCherry) for the detection of estrogenic effects. The protocol covers the propagation of the fish and treatment of embryos, and emphasizes the detection, documentation, and the evaluation of fluorescent signals induced by endocrine disrupting compounds (EDC).

Explore More Videos

Tg(Vtg1:mcherry) ZebrafishEndocrine Disrupting CompoundsEstrogenic EffectsBiomarkerVitellogeninIn Vivo TestingEnvironmental SamplesAnimal Welfare

Read Article

Cite this Article

Csenki, Z., Horváth, Á., Bock, I., Garai, E., Kerekes, F., Vásárhelyi, E., Kovács, B., Urbányi, B., Mueller, F., Bakos, K. Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds. J. Vis. Exp. (162), e60462, doi:10.3791/60462 (2020).

Download .ris file

Copy

Share Video

.

Copy