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Biochemie
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High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
JoVE Journal
Biochemie
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JoVE Journal Biochemie
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

DOI:
10.3791/60096-v

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09:33 min

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October 15, 2019

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, , , , ,
1Institute of Physiology, Faculty of Medicine,University of Freiburg, Freiburg, Germany, 2Institute of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine,University of Freiburg, Freiburg, Germany, 3Signalling Research Centres BIOSS and CIBSS, Freiburg, Germany, 4Logopharm GmbH, Germany

Kapitel

  • 00:04Titel
  • 01:16Preparative BN-PAGE
  • 03:44Gel Sampling and Digestion
  • 06:32Results: High-Resolution Complexome Profiling
  • 08:29Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

A versatile cryoslicing BN-MS protocol using a microtome is presented for high-resolution complexome profiling.

Tags

CsBN-MSProtein ComplexesMembrane ProteinsSignal TransductionNative Protein ComplexSubunit CompositionGradient GelPolymerizationLoading SlotsRunning BuffersProtein Solubilization

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