Journal
/
Entwicklungsbiologie
/
Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon

Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon

9,429 Views

•

06:02 min

•

September 13, 2019

DOI:

10.3791/60066-v

DOI:
10.3791/60066-v

•

06:02 min
September 13, 2019

•

4 Views
,
1Institute of Stem Cell Research,Helmholtz Zentrum München, 2Graduate School of Systemic Neurosciences,Ludwig Maximilian University, 3Department of Cell Biology and Anatomy, Biomedical Center,Ludwig Maximilian University, 4Munich Cluster for Systems Neurology (SyNergy)

Summary

Automatically generated

Presented here is an electroporation method for plasmid DNA delivery and ependymoglial cell labeling in the adult zebrafish telencephalon. This protocol is a quick and efficient method to visualize and trace individual ependymoglial cells and opens up new possibilities to apply electroporation to a broad range of genetic manipulations.

Explore More Videos

ElectroporationPlasmid DNAIn Vivo DeliveryZebrafish TelencephalonEpendymoglial CellsCell LabelingCell-autonomous ManipulationCell BiologyCell DelaminationEpithelial HomeostasisCell Division SymmetryGlass CapillaryMicroinjectionAnesthesiaStereomicroscopeMicroknifeTelencephalic VentricleUltrasound GelElectroporation

Read Article

Cite this Article

Durovic, T., Ninkovic, J. Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon. J. Vis. Exp. (151), e60066, doi:10.3791/60066 (2019).

Download .ris file

Copy

Share Video

.

Copy