Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Stem Cell-Derived Viral Ag-Specific T Lymphocytes …
JoVE Journal
Immunology and Infection

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Immunology and Infection
Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice
 

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice

Article DOI: 10.3791/60043-v • 07:25 min • September 25th, 2019
September 25th, 2019 •
1, 2, 1, 1
1Department of Microbial Pathogenesis and Immunology, Texas A&M University Health Science Center, 2Department of Ophthalmology, Harvard Medical School

Kapitel

Summary
Automatic Translation

Presented here is a protocol for the effective suppression of hepatitis B virus (HBV) replication in mice by utilizing adoptive cell transfer (ACT) of stem cell-derived viral antigen (Ag)-specific T lymphocytes. This procedure may be adapted for potential ACT-based immunotherapy of HBV infection.

Tags

Stem Cell-derived Viral Ag-specific T Lymphocytes Suppress HBV Replication Adoptive Cell Transfer IPSC-derived Viral Antigen-specific T Cell Naive Phenotype Differentiation CD8-positive T Cells Plate S183 T Cell Receptor Gene-transduced IPSCs OP9-DL1/DL4 Monolayer Alpha-minimum Medium Fetal Bovine Serum Murine Flt3 Ligand Co-culture Cell Morphology Supernatant Floating Cells Adherent Cells Trypsin Enzymatic Reaction IPSC Medium Centrifugation Diluted Brefeldin A Intracellular Cytokines
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter