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Immunologie und Infektion

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1차 인간 단핵구의 고립, 형질전환 및 문화

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Immunologie und Infektion

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JoVE Journal Immunologie und Infektion

Isolation, Transfection, and Culture of Primary Human Monocytes

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1차 인간 단핵구의 고립, 형질전환 및 문화

12,652 Views

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09:13 min

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December 16, 2019

DOI:

10.3791/59967-v

DOI:

10.3791/59967-v

•

09:13 min

December 16, 2019

•

3 Views

Karlie Plaisance-Bonstaff², Celeste Faia, Dorota Wyczechowska, Duane Jeansonne, Cecilia Vittori³, Francesca Peruzzi²

¹Department of Medicine,Louisiana State University Health Sciences Center, ²Department of Microbiology, Immunology, and Parasitology,Louisiana State University Health Sciences Center, ³Department of Biomedical and Clinical Sciences L. Sacco,University of Milan

Tags

Primary Human Monocytes Isolation Transfection Culture HIV Infection Immune Dysfunction Antiretroviral Therapy Molecular Mechanisms Biosafety EDTA Magnetic Beads PBS Centrifugation Cell Counting

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