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Genetik
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高密度DNA和RNA微阵列.光刻合成、杂交和制备大型核酸库
JoVE Journal
Genetik
This content is Free Access.
JoVE Journal
Genetik
High-Density DNA and RNA microarrays – Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
Please note that all translations are automatically generated.
Click here for the English version.
高密度DNA和RNA微阵列.光刻合成、杂交和制备大型核酸库
DOI:
10.3791/59936-v
•
11:22 min
•
August 12, 2019
•
Jory Lietard
,
Erika Schaudy
,
Kathrin Hölz
,
Dominik Ameur
,
Mark M. Somoza
1
Institute of Inorganic Chemistry, Faculty of Chemistry
,
University of Vienna
Kapitel
00:05
Titel
00:54
Preparation and Monitoring of Microarray Synthesis
04:26
DNA and RNA Microarray Deprotection
06:26
Hybridization with a Fluorescently-Labelled Complementary Strand
08:09
Library Deprotection, Cleavage, and Recovery
09:11
Results: Hybridization Assays to the 25mer DNA and RNA Sequences Synthesized In Situ on Microarrays
10:31
Conclusion
Summary
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Automatische Übersetzung
本文提出并讨论了原位制造核酸微阵列的合成和应用的新进展。具体来说,我们展示了DNA合成的规程如何扩展到RNA,以及如何利用微阵列创建可检索的核酸库。
Tags
DNA Microarray
RNA Microarray
Photolithography
In Situ Synthesis
Chemically Modified Oligonucleotides
Solid Phase DNA Synthesis
UV LED
Intensity Meter
WiCell Controller Software
Micromere Device
Gasket
Microscope Slide
PTFE Gasket
Double-substrate Cell
Torque Screwdriver
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