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High-Density DNA and RNA microarrays – Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
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High-Density DNA and RNA microarrays – Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays – Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

17,062 Views

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11:22 min

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August 12, 2019

DOI:

10.3791/59936-v

DOI:
10.3791/59936-v

•

11:22 min
August 12, 2019

•

15 Views
, , , ,
1Institute of Inorganic Chemistry, Faculty of Chemistry,University of Vienna

概要

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In this article, we present and discuss new developments in the synthesis and applications of nucleic acid microarrays fabricated in situ. Specifically, we show how the protocols for DNA synthesis can be extended to RNA and how microarrays can be used to create retrievable nucleic acid libraries.

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Cite this Article

Lietard, J., Schaudy, E., Hölz, K., Ameur, D., Somoza, M. M. High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries. J. Vis. Exp. (150), e59936, doi:10.3791/59936 (2019).

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