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Immunologie und Infektion

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高产细胞外囊泡制剂的纯化远离病毒

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Immunologie und Infektion

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JoVE Journal Immunologie und Infektion

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Please note that all translations are automatically generated. Click here for the English version.

高产细胞外囊泡制剂的纯化远离病毒

DOI:

10.3791/59876-v

•

00:07 min

•

September 12, 2019

•

Catherine DeMarino*¹, Robert A. Barclay*¹, Michelle L. Pleet, Daniel O. Pinto, Heather Branscome², Siddhartha Paul, Benjamin Lepene, Nazira El-Hage, Fatah Kashanchi

¹Laboratory of Molecular Virology, School of Systems Biology,George Mason University, ²American Type Culture Collection (ATCC), ³ATCC Cell Systems, ⁴Ceres Nanosciences, Inc, ⁵Department of Immunology and Nano-medicine, Herbert Wertheim College of Medicine,Florida International University

Kapitel

00:04Titel
01:00Filtration and Precipitation of Extracellular Vesicles
02:25Construction of a Density Gradient and Enrichment of EV Fractions
04:06Preparation of Nanoparticle Pellet for Downstream Assay
05:05Results: Increased EV Yield and Characterization of EVs
06:09Conclusion

Summary

Automatische Übersetzung

该协议通过结合EV沉淀、密度梯度超离和颗粒捕获，将细胞外囊泡（EV）与高效和屈服的病毒分离，从而简化工作流程和减少启动体积要求，导致可重复的制剂用于所有EV研究。

Tags

Extracellular Vesicles EVs Virus-free EV Preparations EV Purification Ultracentrifugation PEG Precipitation Iodixanol Density Gradient Nanoparticle Enrichment

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