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量化龙脑中的亚细胞泛基蛋白-蛋白酶体活性
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JoVE Journal Neurowissenschaften
Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain
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量化龙脑中的亚细胞泛基蛋白-蛋白酶体活性

6,519 Views

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09:25 min

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May 21, 2019

DOI:

10.3791/59695-v

DOI:
10.3791/59695-v

•

09:25 min
May 21, 2019

•

26 Views
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1Department of Animal and Poultry Sciences,Virginia Polytechnic Institute and State University, 2School of Neuroscience,Virginia Polytechnic Institute and State University

Summary

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该协议旨在有效地量化啮齿动物大脑不同细胞舱中的泛泛蛋白-蛋白酶体系统(UPS)活性。用户能够检查UPS在同一动物中核、细胞质和突触分数的功能,从而减少执行这些复杂分析所需的时间和动物数量。

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Cite this Article

McFadden, T., Devulapalli, R. K., Jarome, T. J. Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain. J. Vis. Exp. (147), e59695, doi:10.3791/59695 (2019).

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