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原子力显微镜对细胞外囊泡的成像

JoVE Journal
Biochemie

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JoVE Journal Biochemie

Imaging of Extracellular Vesicles by Atomic Force Microscopy

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原子力显微镜对细胞外囊泡的成像

12,550 Views

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10:11 min

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September 11, 2019

DOI:

10.3791/59254-v

DOI:

10.3791/59254-v

•

10:11 min

September 11, 2019

•

38 Views

Mikhail Skliar^1,2, Vasiliy S. Chernyshev^3,4

¹Department of Chemical Engineering,University of Utah, ²The Nano Institute of Utah,University of Utah, ³Center for Photonics and Quantum Materials,Skolkovo Institute of Science and Technology, ⁴Biopharmaceutical Cluster ‘Northern’,Moscow Institute of Physics and Technology

Summary

描述了一个分步程序，用于从液体样品中分离体和细胞外囊泡的无标签固定，并通过原子力显微镜（AFM）进行成像。AFM 图像用于估计溶液中囊泡的大小，并描述其他生物物理特性。

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