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Proteoom bestrijkende kwantificering van Labeling homogeniteit op het niveau van één molecuul
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Biochemie
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JoVE Journal Biochemie
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
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Proteoom bestrijkende kwantificering van Labeling homogeniteit op het niveau van één molecuul

5,979 Views

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08:29 min

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April 19, 2019

DOI:

10.3791/59199-v

DOI:
10.3791/59199-v

•

08:29 min
April 19, 2019

•

1 Views
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1Laboratory for Cell Systems Control, RIKEN Center for Biosystems Dynamics Research,RIKEN, 2Laboratoire de Biomatériaux et Bioimagerie,INSERM, 3PRESTO, Japan Science and Technology Agency

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Protein QuantificationSingle-molecule AnalysisFluorescent LabelingHeterogeneityMolecular Concentration AssaysDiagnostic MethodsProtein Species AnalysisData ReproducibilityLysing BufferTween-20DTTCHAPSCy3 NHS Ester DyeHEPES-NaOHBiotin-2-amineEDC

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Leclerc, S., Arntz, Y., Taniguchi, Y. Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level. J. Vis. Exp. (146), e59199, doi:10.3791/59199 (2019).

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