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一种高通量志贺氏菌特异性杀菌试验
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
A High-throughput Shigella-specific Bactericidal Assay
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一种高通量志贺氏菌特异性杀菌试验

11,185 Views

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06:11 min

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February 27, 2019

DOI:

10.3791/59164-v

DOI:
10.3791/59164-v

•

06:11 min
February 27, 2019

•

7 Views
, , ,
1Department of Enteric Infections, Bacterial Diseases Branch,Walter Reed Army Institute of Research, 2University of Alabama at Birmingham

Summary

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在这里, 我们提出了一个方案来测量血清中抗体的志贺酸酯活性。血清与细菌和外源补体混合, 孵育, 反应混合物被镀在琼脂板上。活细菌形成菌落, 这些菌落被计数, 使用自动菌落计数器, 并用于确定杀菌滴度。

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Cite this Article

Weerts, H. P., Yu, J., Kaminski, R. W., Nahm, M. H. A High-throughput Shigella-specific Bactericidal Assay. J. Vis. Exp. (144), e59164, doi:10.3791/59164 (2019).

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