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MicroFocus X-ray CT (microCT) Imaging af Actinia equina (CNIDARIA), Harmothoe Sp. (Annelida), og xenoturbella japonica (xenacoelomorpha)
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Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)

MicroFocus X-ray CT (microCT) Imaging af Actinia equina (CNIDARIA), Harmothoe Sp. (Annelida), og xenoturbella japonica (xenacoelomorpha)

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08:09 min

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August 06, 2019

DOI:

10.3791/59161-v

DOI:
10.3791/59161-v

•

08:09 min
August 06, 2019

•

3 Views
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1Mammalian Genetics Laboratory,National Institute of Genetics, 2Misaki Marine Biological Station,The University of Tokyo, 3Shimoda Marine Research Center,University of Tsukuba

Summary

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Her forklares i detaljer protokoller til udførelse af MicroFocus X-ray computertomografi (microCT) Imaging af tre Marine hvirvelløse dyr. Denne undersøgelse beskriver trin som prøve fiksering, farvning, montage, scanning, billed rekonstruktion og dataanalyser. Der gives også forslag til, hvordan protokollen kan justeres for forskellige prøver.

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MicroCTActinia EquinaHarmothoe Sp.Xenoturbella JaponicaMarine InvertebratesSample MountingAgaroseStainingSample Preparation

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Maeno, A., Kohtsuka, H., Takatani, K., Nakano, H. Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha). J. Vis. Exp. (150), e59161, doi:10.3791/59161 (2019).

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