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免疫荧光定量小鼠脑突触蛋白的异种分布
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JoVE Journal Neurowissenschaften
Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence
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免疫荧光定量小鼠脑突触蛋白的异种分布

7,684 Views

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09:18 min

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January 29, 2019

DOI:

10.3791/58940-v

DOI:
10.3791/58940-v

•

09:18 min
January 29, 2019

•

5 Views
, ,
1Institute of Anatomy and Embryology,University Medical Center Göttingen

Summary

Automatically generated

在这里, 我们描述了一种定量的方法来确定突触蛋白相对于标记蛋白使用免疫荧光染色, 共聚焦显微镜, 和基于计算机的分析。

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ImmunofluorescenceConfocal MicroscopyProtein DistributionHeterogeneityMouse BrainCryo-protectionCryosectioningBrain SlicesImmunostainingQuantification

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Cite this Article

Wallrafen, R., Dresbach, T., Viotti, J. S. Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence. J. Vis. Exp. (143), e58940, doi:10.3791/58940 (2019).

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