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Immunology and Infection

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JoVE Journal Immunology and Infection
Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
 

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Article DOI: 10.3791/58689-v • 09:40 min • November 28th, 2018
November 28th, 2018 •
1,2, 1, 1
1Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 2ORAU/ORISE

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Summary
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Here, we present a protocol optimized for the processing of coding (mRNA) and non-coding (ncRNA) globin reduced RNA-seq libraries from a single whole blood sample.

Tags

Coding RNA Non-coding RNA Viral RNA Host-pathogen Interactions Host Amino Response Biological Functions MRNA Globin-reduced RNAseq Libraries Centrifuging RNase-free Water Pellet Total RNA Isolation Lysis Binding Buffer Homogenate Additive MiRNA Isolation Kit
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