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RNA-baserade omprogrammering av mänskliga primära fibroblaster till inducerade pluripotenta stamceller
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
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RNA-baserade omprogrammering av mänskliga primära fibroblaster till inducerade pluripotenta stamceller

9,864 Views

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11:38 min

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November 26, 2018

DOI:

10.3791/58687-v

DOI:
10.3791/58687-v

•

11:38 min
November 26, 2018

•

2 Views
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1Department of Dermatology,University of Colorado School of Medicine, Anschutz Medical Campus, 2Charles C. Gates Center for Regenerative Medicine,University of Colorado School of Medicine, Anschutz Medical Campus, 3Stem Cell Biobank and Disease Modeling Core,University of Colorado School of Medicine, Anschutz Medical Campus

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RNA-based ReprogrammingInduced Pluripotent Stem CellsIPSCsPrimary FibroblastsTransfection EfficiencyPH AdjustmentLaminin 521Fibroblast Expansion MediumPlating MediumFGFB18R

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McGrath, P. S., Diette, N., Kogut, I., Bilousova, G. RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells. J. Vis. Exp. (141), e58687, doi:10.3791/58687 (2018).

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