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An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues

An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues

DOI:
10.3791/58476-v

•

07:50 min

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January 07, 2019

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, , , , , , , , , ,
1Cardiovascular Developmental Biology, Department of Cardiovascular Sciences,KU Leuven, 2Centre for Molecular and Vascular Biology, Department of Cardiovascular Sciences,KU Leuven, 3Department of Biohybrid & Medical Textiles, AME – Helmholtz Institute for Biomedical Engineering,RWTH Aachen University, 4European Homograft Bank,Saint Jean Clinique, 5Division of Clinical Cardiac Surgery, Department of Cardiovascular Sciences,KU Leuven

Kapitel

  • 00:04Titel
  • 01:04In vitro Perfusion Experiments using Parallel-Plate Flow Chamber
  • 03:34Serial Dilution on Mueller-Hinton Blood Agar Plates
  • 06:28Results: Direct Evaluation of Bacterial Adhesion
  • 07:09Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We describe an in-house designed in vitro flow chamber model, which allows the investigation of bacterial adherence to graft tissues.

Tags

In Vitro ModelParallel-plate Perfusion SystemBacterial AdherenceInfective EndocarditisPathogenesisBacteriaBlood CellsEndothelial CellsFlow ConditionsVegetation FormationVascular PermeabilityCell MobilityGene ExpressionBacterial SuspensionShear StressPeristaltic PumpFluorescently-labeled Colony Forming UnitsPhosphate Buffered Saline

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