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An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues
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07:50 min
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January 07, 2019
DOI:
1Cardiovascular Developmental Biology, Department of Cardiovascular Sciences,KU Leuven, 2Centre for Molecular and Vascular Biology, Department of Cardiovascular Sciences,KU Leuven, 3Department of Biohybrid & Medical Textiles, AME – Helmholtz Institute for Biomedical Engineering,RWTH Aachen University, 4European Homograft Bank,Saint Jean Clinique, 5Division of Clinical Cardiac Surgery, Department of Cardiovascular Sciences,KU Leuven
Summary
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We describe an in-house designed in vitro flow chamber model, which allows the investigation of bacterial adherence to graft tissues.
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In Vitro ModelParallel-plate Perfusion SystemBacterial AdherenceInfective EndocarditisPathogenesisBacteriaBlood CellsEndothelial CellsFlow ConditionsVegetation FormationVascular PermeabilityCell MobilityGene ExpressionBacterial SuspensionShear StressPeristaltic PumpFluorescently-labeled Colony Forming UnitsPhosphate Buffered Saline
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Cite this Article
Ditkowski, B., Veloso, T. R., Bezulska-Ditkowska, M., Lubig, A., Jockenhoevel, S., Mela, P., Jashari, R., Gewillig, M., Meyns, B., Hoylaerts, M. F., Heying, R. An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues. J. Vis. Exp. (143), e58476, doi:10.3791/58476 (2019).
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