Mate-guarding behavior plays an important role in reproduction of intertidal copepods of the genus Tigriopus. However, methods for studying this behavior have not been well described. Here we describe methods for: 1) individual culture of virgin Tigriopus animals, and 2) quantitative analysis of their mate-guarding behavior.
Tsuboko-Ishii, S., Burton, R. S. Individual Culturing of Tigriopus Copepods and Quantitative Analysis of Their Mate-guarding Behavior. J. Vis. Exp. (139), e58378, doi:10.3791/58378 (2018).