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Individual Culturing of Tigriopus Copepods and Quantitative Analysis of Their Mate-guarding Behavior
JoVE 杂志
环境科学
This content is Free Access.
JoVE 杂志 环境科学
Individual Culturing of Tigriopus Copepods and Quantitative Analysis of Their Mate-guarding Behavior
DOI:

06:24 min

September 26, 2018

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Chapters

  • 00:04Title
  • 00:47Preparation of Virgin Animals for Behavioral Observation
  • 02:00Behavioral Test and Video Recording of Mate-Guarding Behavior
  • 02:53Manual Analysis of Behavioral Properties
  • 03:31Two-Dimensional Tracking of Individuals and Pairs
  • 05:17Results: Male-Male pairs of T. californicus Demonstrate Shorter Duration of Pairing and Higher Velocity Compared to Male-Female Pairs
  • 06:01Conclusion

Summary

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Mate-guarding behavior plays an important role in reproduction of intertidal copepods of the genus Tigriopus. However, methods for studying this behavior have not been well described. Here we describe methods for: 1) individual culture of virgin Tigriopus animals, and 2) quantitative analysis of their mate-guarding behavior.

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