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Visualiseren van Drosophila been Motor Neuron axonen via de volwassen cuticula
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle
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Visualiseren van Drosophila been Motor Neuron axonen via de volwassen cuticula

9,052 Views

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08:33 min

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October 30, 2018

DOI:

10.3791/58365-v

DOI:
10.3791/58365-v

•

08:33 min
October 30, 2018

•

8 Views
, , , ,
1Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, 2Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute,Columbia University, 3Neuroscience Program,NYU School of Medicine

Summary

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Hier beschrijven we een protocol om te visualiseren de axonale richt met een TL proteïne in volwassen benen van Drosophila door fixatie, montage, imaging en na imaging stappen.

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Guan, W., Venkatasubramanian, L., Baek, M., Mann, R. S., Enriquez, J. Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle. J. Vis. Exp. (140), e58365, doi:10.3791/58365 (2018).

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