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In Vitro Differenziazione dei Mouse del Granulocyte-macrofago-colony-stimulating Factor (GM-CSF)-producendo le cellule T Helper (THGM)
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells
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In Vitro Differenziazione dei Mouse del Granulocyte-macrofago-colony-stimulating Factor (GM-CSF)-producendo le cellule T Helper (THGM)

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10:27 min

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September 10, 2018

DOI:

10.3791/58087-v

DOI:
10.3791/58087-v

•

10:27 min
September 10, 2018

•

1 Views
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1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 2Immunology Programme, Life Sciences Institute,National University of Singapore, 3Cancer Science Institute of Singapore, Yong Loo Lin School of Medicine,National University of Singapore

Summary

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Qui, presentiamo un protocollo per differenziare murino granulocyte-macrophage-colony-stimulating-factor-producing linfociti T helper (THGM) da cellule T CD4 + ingenuo, incluso l'isolamento delle cellule T CD4 + Naive, differenziazione di THGM, e analisi delle cellule di THGM differenziate. Questo metodo può essere applicato agli studi del regolamento e la funzione delle cellule di THGM.

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Cite this Article

Lu, Y., Fu, X., Zhang, Y. In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells. J. Vis. Exp. (139), e58087, doi:10.3791/58087 (2018).

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