/
/
Synaptic dataloger modellering med 3D Cocultures astrocytter og neuroner fra humane pluripotente stamceller
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Kapitel
- 00:04Titel
- 00:49Generation and Maintenance of Astrocyte Progenitors (hAstros) from hPSCs
- 02:41Generation and Maintenance of Neurons (hNeurons) and Inducible Neurons (iNeurons) from hPSCs
- 03:27Preparation and Maintenance of 3D Sphere Co-cultures
- 05:42Measurement of Live Synaptic Physiology with Multielectrode Arrays (MEAs)
- 06:56Results: Morphological and Physiological Characteristics of Synaptic Microcircuits
- 08:07Conclusion
I denne protokol, vi har til formål at beskrive en reproducerbar metode til at kombinere dissocieres humane pluripotente stamceller afledt neuroner og astrocytter sammen i 3D sfære cocultures, vedligeholdelse af disse områder i frie flydende betingelser, og efterfølgende måle synaptic kredsløb aktivitet af kugler med immunoanalysis og multielectrode array optagelser.
Tags
Synaptic Microcircuit3D CoculturesAstrocytesNeuronsHuman Pluripotent Stem CellsNeural Cell TypesSynaptic Circuit FormationNeurowissenschaftenOrganoid TechnologiesDrug DevelopmentNeuroregenerationNeural ProgenitorsAstrocyte ProgenitorsAstrocyte SubtypesNeural InductionNeural MediumRho-kinase InhibitorECM-coated PlatesCell AggregationCell DissociationCell Identity ConfirmationCryo-preservation
