/
/
Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells
12,068 Views
•
08:48 min
•
August 16, 2018
DOI:
Summary
Automatically generated
In this protocol, we aim to describe a reproducible method for combining dissociated human pluripotent stem cell derived neurons and astrocytes together into 3D sphere cocultures, maintaining these spheres in free floating conditions, and subsequently measuring synaptic circuit activity of the spheres with immunoanalysis and multielectrode array recordings.
Explore More Videos
Synaptic Microcircuit3D CoculturesAstrocytesNeuronsHuman Pluripotent Stem CellsNeural Cell TypesSynaptic Circuit FormationNeurociênciaOrganoid TechnologiesDrug DevelopmentNeuroregenerationNeural ProgenitorsAstrocyte ProgenitorsAstrocyte SubtypesNeural InductionNeural MediumRho-kinase InhibitorECM-coated PlatesCell AggregationCell DissociationCell Identity ConfirmationCryo-preservation
Read Article
Cite this Article
Cvetkovic, C., Basu, N., Krencik, R. Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells. J. Vis. Exp. (138), e58034, doi:10.3791/58034 (2018).
Copy