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Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells
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08:48 min
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August 16, 2018
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In this protocol, we aim to describe a reproducible method for combining dissociated human pluripotent stem cell derived neurons and astrocytes together into 3D sphere cocultures, maintaining these spheres in free floating conditions, and subsequently measuring synaptic circuit activity of the spheres with immunoanalysis and multielectrode array recordings.
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Synaptic Microcircuit3D CoculturesAstrocytesNeuronsHuman Pluripotent Stem CellsNeural Cell TypesSynaptic Circuit Formation神经科学Organoid TechnologiesDrug DevelopmentNeuroregenerationNeural ProgenitorsAstrocyte ProgenitorsAstrocyte SubtypesNeural InductionNeural MediumRho-kinase InhibitorECM-coated PlatesCell AggregationCell DissociationCell Identity ConfirmationCryo-preservation
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Cvetkovic, C., Basu, N., Krencik, R. Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells. J. Vis. Exp. (138), e58034, doi:10.3791/58034 (2018).
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