Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Expression and Purification of Mammalian Bestrophin Ion Channels
8,173 Views
•
08:12 min
•
August 02, 2018
DOI:
Summary
Automatically generated
The purification of ion channels is often challenging, but once achieved, it can potentially allow in vitro investigations of the functions and structures of the channels. Here, we describe the stepwise procedures for the expression and purification of mammalian bestrophin proteins, a family of Ca2+-activated Cl- channels.
Read Article
Cite this Article
Kittredge, A., Ward, N., Hopiavuori, A., Zhang , Y., Yang, T. Expression and Purification of Mammalian Bestrophin Ion Channels. J. Vis. Exp. (138), e57832, doi:10.3791/57832 (2018).
Copy