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Expression and Purification of Mammalian Bestrophin Ion Channels
Chapters
- 00:04Title
- 00:43Protein Expression
- 03:00Protein Purification
- 07:02Results: Expression and Purification of a Mammalian Bestrophin Protein
- 07:32Conclusion
The purification of ion channels is often challenging, but once achieved, it can potentially allow in vitro investigations of the functions and structures of the channels. Here, we describe the stepwise procedures for the expression and purification of mammalian bestrophin proteins, a family of Ca2+-activated Cl- channels.
