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线虫线虫中乳酸和丙酮酸细胞的小尺度比色测定
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Biochemie
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JoVE Journal Biochemie
Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans
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线虫线虫中乳酸和丙酮酸细胞的小尺度比色测定

6,750 Views

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09:35 min

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October 15, 2018

DOI:

10.3791/57807-v

DOI:
10.3791/57807-v

•

09:35 min
October 15, 2018

•

1 Views
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1Department of Health Science, School of Sports & Health Science,Daito Bunka University, 2Department of Molecular Life Science, School of Medicine,Tokai University, 3Department of Health Management, School of Health Study,Tokai University

Tags

Colorimetric AssayLactatePyruvateCaenorhabditis ElegansNematodeProtein PrecipitationHomogenizationE. Coli OP50NGM Agar PlatesGravid HermaphroditesEgg IsolationL1 LarvaeSynchronous CultureColorimetric AnalysisBiochemical Methodology

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Yanase, S., Yasuda, K., Ishii, N. Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans. J. Vis. Exp. (140), e57807, doi:10.3791/57807 (2018).

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