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Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models

Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models

DOI:
10.3791/57795-v

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11:29 min

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September 06, 2018

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, , , ,
1Institut de la Vision,Sorbonne Université, INSERM, CNRS, F-75012 Paris, France

Kapitel

  • 00:04Titel
  • 01:04Generation and Maturation of Retinal Organoids
  • 02:50Generation and Amplification of Human iPSC-derived RPE (hiRPE) Cells
  • 05:17Cryopreservation of Retinal Organoids and hiRPE Cells
  • 07:09Thawing of Retinal Organoids and hiRPE Cells
  • 09:01Results: Generation of All Neural Retina Cell Types and RPE
  • 10:19Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The production of specialized retinal cells from pluripotent stem cells is a turning point in the development of stem cell-based therapy for retinal diseases. The present paper describes a simple method for an efficient generation of retinal organoids and retinal pigmented epithelium for basic, translational, and clinical research.

Tags

IPS CellsRetinal Cell ModelsRetinal OrganoidsRetinal Pigmented Epithelial CellsRPE CellsXeno-freeFeeder-freeDifferentiationCulture ConditionsCell IsolationFloating CulturePro B-27 MediumPro N-2 MediumFGF2

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