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A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

DOI:
10.3791/57784-v

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06:17 min

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August 25, 2018

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, , , , , ,
1Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology,Shandong University, 2Suzhou Institute of Shandong University, 3Department of Urology,Qilu Hospital of Shandong University

Kapitel

  • 00:00Titel
  • 00:20Skin Tissue Pretreatment
  • 01:07Digestion of skin tissue
  • 02:11Collecting and culturing primary cells
  • 03:42Cell Passage
  • 04:46Representative results
  • 06:06Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here we present a protocol to efficiently isolate primary human keratinocytes from adult skin tissues. This method simplifies the conventional procedure by using the ROCK Inhibitor Y-27632 in the inoculation medium to spontaneously separate epidermal cells from dermal cells.

Tags

Primary Human KeratinocytesIsolationCultureSimplified MethodEfficientEpidermal CellsLaboratory ApplicationsClinical ApplicationsHomogenizationEnzymatic DigestionTrypsinDNase ICell StrainerCell CultureROCK InhibitorKeratinocyte MediumPassaging

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