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Birincil insan Lenfositi yetişkin cilt dokusundan izole etmek için basit ve etkili bir yöntem
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
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Birincil insan Lenfositi yetişkin cilt dokusundan izole etmek için basit ve etkili bir yöntem

9,723 Views

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06:17 min

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August 25, 2018

DOI:

10.3791/57784-v

DOI:
10.3791/57784-v

•

06:17 min
August 25, 2018

•

10 Views
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1Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology,Shandong University, 2Suzhou Institute of Shandong University, 3Department of Urology,Qilu Hospital of Shandong University

Summary

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Burada birincil insan Lenfositi yetişkin cilt dokularının üzerinden verimli bir şekilde ayırmak için bir protokol mevcut. Bu yöntem geleneksel yordamı ROCK inhibitörü Y-27632 aşılama ortamda kendiliğinden dermal hücrelerden epidermal hücrelerin ayırarak basitleştirir.

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Primary Human KeratinocytesIsolationCultureSimplified MethodEfficientEpidermal CellsLaboratory ApplicationsClinical ApplicationsHomogenizationEnzymatic DigestionTrypsinDNase ICell StrainerCell CultureROCK InhibitorKeratinocyte MediumPassaging

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Cite this Article

Liu, Z., Wen, J., Leng, X., Zhou, Q., Zhou, C., Zhao, H., Wu, X. A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue. J. Vis. Exp. (138), e57784, doi:10.3791/57784 (2018).

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